Then trickle a little Biuret solution B down the. You'll need to be able to describe how and why each step in the emulsion test is done and how to interpret the results. A brick-red precipitate indicates that reducing sugars are present. Understand also that a Bunsen burn is NOT needed for this procedure. However, this component is almost a sure-hit for Science Biology practical exams, and with the short duration of an estimated 45 minutes to complete the Biology section, these just might help you save a couple of minutes. Equipment. It's positive if there's a brick-red precipitate. Biology is brought to you with support from the. Study with Quizlet and memorize flashcards containing terms like test for sugars, Test for starch, Test for protein and more. What is happening to the copper (II) ions in the Benedicts test? All of these chemical tests can be learnt, but its even better if the chemistrybehind them is understood. Find the ratio of the Sun's tidal force per unit mass on Mercury at perihelion to the Sun's tidal force per unit mass on Earth. I have uploaded a one-page handout for, This is my favourite food test to observe cos' it yields so pretty, For this test, there is another way besides the one I've indicated in the resource handout. This provides an introduction to the idea that food is composed of different substances and this can then lead to a discussion of what the nutritional label tells us about what else is in cereal. REMEMBER: If you use a stirring rod, wash it after every use, so you wont contaminate one solution with another. Lipids. This property of dissolving fats in alcohol is the basis of the emulsion test. Here, you can browse videos, articles, and exercises by topic. The negative control remained blue while the positive control turned from blue to orange with precipitatae. Place pea-sized portions of glucose and the unknown substance you are testing in separate test tubes. However, this component is almost a sure-hit for Science Biology practical exams, and with the short duration of an estimated 45 minutes to complete the Biology section, these. Use tab to navigate through the menu items. And when it finally boils, she (in today's post, for simplicity's sake, the student will be a "she") places 3 test tubes in there, but the boiling water threatens to spill over because there's just too much water in the beaker. Ask me if you need help for GCSE revision? Discover short videos related to how to remember food tests in biology on TikTok. For the other food types, first prepare a sample of food for testing: 1. Current Year 10 Official Thread (2022-2023). food tests biology. Test for proteins Biuret test - add sodium hydroxide solution to the sample. Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. Filter the solution using a funnel and filter paper (to remove the solid bits of food left over) Now you can use the final solution for the food tests. ": TLG's PhD study blog! The simpler method is to just add 2ml of Biuret solution to 2ml of food sample and shake. Typically, people . Current Year 10 Official Thread (2022-2023). Alternatively, I've also shared it in my Telegram channel for easy access on your mobile. 1.3.3 Diffusion in Multicellular Organisms, 2.2 Organisation: The Cardiovascular & Respiratory System, 2.3.3 Lifestyle & Non-Communicable Diseases, 2.3.4 Data & Applications of Lifestyle Disease, 3.3.1 Detection and Identification of Plant Diseases, 4.1.4 Required Practical: Photosynthesis Rate, 4.1.5 Uses of Glucose from Photosynthesis, 5.2 Hormones: Maintaining Blood Homeostasis, 5.2.3 Control of Blood Glucose Concentration, 5.2.4 Maintaining Water & Nitrogen Balance in the Body, 5.3 Hormones in Humans: Reproduction & Metabolism, 5.3.3 The Uses of Hormones to Treat Infertility, 6.1.3 Advantages & Disadvantages of Sexual & Asexual Reproduction, 6.3 The Development of Understanding of Genetics & Evolution, 7.1 Adaptations, Interdependence & Competition, 7.3 Biodiversity & the Effect of Human Interaction on Ecosystems, Aim: To use qualitative reagents to test for a range of carbohydrates, lipids and proteins. Add Benedict's reagent to the sample. Break up your food using a mortar and pestle. Control the boiling by turning the hot plate on and off as needed. To test for starch in either a food sample or a leaf, simply add iodine solution to the sample. Carefully heat the test tubes by suspending in a hot water bath using a 500 ml beaker at about 40-50 degrees Celsius for five minutes. science pearson edexcel end of unit test higher. Allow time for the sample to dissolve in the ethanol. The apple contained both starch and sugar as it tested positive for both the iodine test (orange blue - black) and the benedict's test (blue orange). 806 8067 22 Registered Office: Imperial House, 2nd Floor, 40-42 Queens Road, Brighton, East Sussex, BN1 3XB, Taking a break or withdrawing from your course, Official UCL 2023 Undergraduate Applicants Thread, Official Dental Hygiene and Therapy (Oral Health Science) 2023 Entry Thread, 2023 Deloitte Bright start apprenticeship. Reply 1 4 years ago A I just learnt them today aha idk whether I'll remember them but I'll try give something a go now. You should use a special type of water called distilled water - this is pure water that contains no other chemical substances. Use a clean dropper to add 10 drops of iodine-potassium iodide solution to each test tube. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students. 3. Only when students have completed a suitable table can they carry out the experiment. Special consideration due to my sister being in the psych ward? Terms in this set (22) test for sugars. 2. Observations are essential in this practical; you are looking for colour changes in particular which can indicate if a substance is present or absent: Before you can carry out any of the food tests described below, you may need to prepare a food sample first (especially for solid foods to be tested), Break up the food using a pestle and mortar, Transfer to a test tube and add distilled water, Mix the food with the water by stirring with a glass rod, Filter the mixture using a funnel and filter paper, collecting the solution. The Student Room and The Uni Guide are trading names of The Student Room Group Ltd. Register Number: 04666380 (England and Wales), VAT No. I am determined to get 8's and 9's at GCSE. I want to be an Architect, what GCSEs should I do? 6. GCSE worksheet on testing for carbohydrates, lipids and proteins. Food tests: analysisWrite a conclusion to state which food groups are present one of the food samples you tested and an explanation of how you know this. ID: 1490366 Language: English School subject: Biology Grade/level: GCSE Age: 14-16 Main content: Common food tests Other contents: Protein, sugar, lipid, starch, Benedict's, Biuret, iodine, ethanol Add to my workbooks (7) Download file pdf Embed in my website or blog Add to Google Classroom Join the course to watch the video lessons in full and download the accompanying work booklets. Learn. Use a magnet to extract iron metal from a packet of iron fortified cereals. Shake the test tube. I am determined to get 8's and 9's at GCSE. Both solutions remained clear, proving that no fats are present. Use qualitative reagents to test for the presence of key biological molecules in a range of foods. This test has a number of things I want to highlight and is the main reason for this entire post. How could you make each food test quantitative? We then repeated the same steps with egg white solution and our food solutions. Biuret - detects protein - blue to purple iodine - detects starch - orange to blue black. By comparing the color change a reagent produces in the unknown with the change it produces in the known nutrient, you can estimate the amount of that nutrient. Qualitative tests for foods There are several qualitative tests for food chemicals. Why does starch produce a negative result with Benedicts solution unless acid is added? A. brarje. The simple context of urine tests helps students to understand the rationale behind wanting to test for different food groups. Some things to take note of: Only two drops of iodine solution needed. Introduction to macromolecules. Then, we added in 1% copper (ll) sulfate solution drop by drop and mixed it. science pearson edexcel end of unit test higher. Do remember to shake the test tube after every drop you add in. [CDATA[ Test for lipids. Add copper sulphate solution. 3) Gently shake the tube. I've annotated the points in the diagram below so it's easier to follow. I have uploaded a one-page handout for Food Tests under the Resources section which you can download for your reference. The colour should change to violet within five drops if protein is present and if it doesn't, it is likely that it just doesn't contain any protein, so there's no need to add and add like a total of 20 drops! amylase and collagen, fats e.g. We put the equal amount of distilled water and Benedicts Solution and shook it to obtain the negative control set-up. Lastly, we repeated the same steps, subsituting the oil for our 2 different food solutions. A Having trouble remembering the food tests so anything like an anagram would be helpful thanks. And yes, it's iodine solution, not just iodine. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); Your email address will not be published. In both set-ups, the iodine solution changed from brown to blue black, proving that there is some starch present in both potatoes and turnips. Use the white tile provided if it is a solid piece of food sample. Are there any good sites for trace tables n computer science revision? Follow the steps and do use a, There are additional steps to take if the. 2 of us, Tricia and Ru Xuan, conducted our experiment using potato, while the remaining 2 of us, Ni Kher and Xin Ling, used the healthier alternative, turnip for the experiment. Then, we added in more distilled water. In the presence of starch, iodine will turn from brown to blue-black. Flashcards. Official Cambridge Postgraduate Applicants 2023 Thread, my bf gets mad cuz i wont give him bum sex, Barts and the London (QMUL) A100 2023 Entry, A level history ocr French Revolution 2022 paper, Sutton Trust US Programme Cohort 12 (2023) Applicants Thread, Brownies, books and planning a wedding - your favourite fiancs 2nd blog , Official Royal Holloway 2023 Applicants Thread. Lastly, we did the Alcohol Emulsion Test. There are four important types of large organic molecule in living organisms proteins, carbohydrates (sugars & starches), lipids (fats), and nucleic acids. Carbohydrates. When the water boils, use tongs to place the test tubes in the water bath. A-Level Biology "Lipids: The Emulsion Test"Here's a summary of the lesson: -Lipids (fats and oils) are insoluble in water, but they do readily dissolve in ethanol. Donate or volunteer today! Often, though, a table like the one below (Source: 5118/05/O/N/11 paper) is provided and you are only required to write the names of the reagents used so, learn them well and the marks (usually a total of between 2 to 6 marks) will be yours. The simpler method is to just, potassium sodium tartrate (no need to know this but it is to maintain the solubility of copper (II) ions in alkaline solution), The ethanol-emulsion test is fairly simple to do. If you're seeing this message, it means we're having trouble loading external resources on our website. John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine, Organizational Behavior: Managing People and Organizations, Jean Phillips, Ricky W. Griffin, Stanley Gully. First, we added the same amount of distilled water and ethanol solution and shook it thoroughly. Food Tests. A simple investigation can be conducted to investigate the energy content of a food sample. Add 2ml of distilled water into the decanted ethanol and record your observations. Use a clean dropper to add 10 drops of Sudan III solution to each test tube. Lipids are present if a white emulsion appears. Add an. IBO was not involved in the production of, and does not endorse, the resources created by Save My Exams. Place the crushed food into a test tube and add about 2ml of ethanol. food solution is dissolved in isopropyl alcohol = 3; Greasy spot clearly seen when rubbed on brown paper = 5 Results Reagent tests of the foods selected for this lab show that each food tests positive for at least three of the nutrients described above (see Table 2). Animal Coordination, Control & Homeostasis, 7.1.6 Hormones & Assisted Reproductive Technology, 7.2.9 Regulating Blood Glucose Concentration, 8.1.2 Factors Affecting the Rate of Diffusion, 8.2.2 Practical: Investigating Respiration, 9.4.3 Benefits of Maintaining Biodiversity, Before you can carry out any of the food tests described below, you may need to prepare a food sample first (especially for solid foods to be tested), Break up the food using a pestle and mortar, Transfer to a test tube and add distilled water, Mix the food with the water by stirring with a glass rod, Filter the mixture using a funnel and filter paper, collecting the solution, Take the test tube out of the water bath and observe the colour, A positive test will show a colour change from, We can use iodine to test for the presence or absence of starch in a food sample, Allow time for the sample to dissolve in the ethanol, Strain the ethanol solution into another test tube, Add the ethanol solution to an equal volume of, Whilst carrying out this practical you should try to identify the main hazards and be thinking of ways to reduce harm, The Bunsen burner itself is a hazard due to the open flame. Do not forget that a precipitate (ppt) is formed and must be written in the observation. And that's it for food tests. I hope you find these notes helpful for the upcoming practical exams! Add about 2.5 ml of distilled water and 10 drops of Benedicts solution to each test tube. In this experiment you will evaluate the nutrient content of unidentified food samples. Reducing sugars - benedicts - goes from blue to green to yellow to brick red. Benedict solution-Posotive:Red-Negative:No change. Be sure to label all test tubes. Once students have been introduced to the challenge, ask them to create a results table. Small amounts of glucose will form a yellow or green precipitate. Stir the mixture with a glass rod until you can see some of your food dissolve. This is called the biuret test. Add 10 drops of Benedict's solution to each test tube. At the beginning, starch, glucose solution, egg albumen, peanut oil were labeled A, B, C, and D. The first test was for starch. Public Health ST1 Programme 2023 Entry Thread, The Pupillage Interview/Acceptance/Rejection Thread 2023 Watch. A positive test for fat is a translucent stain around the food sample when you hold the paper up to the light. Record your observations in the DATA TABLE. Question 1 . Repeat step 6 with the unknown substance. Add about 2.5 ml of distilled water and 10 drops of Benedict's solution to each test tube. There are different tests which can be used to detect carbohydrates, proteins and lipids. AQA Biology GCSE - way to remember food tests? . The apple did not contain protein or lipid (fat) as the biuret and emulsion tests were both negative. orkbook with knowledge check and exam style questions with properly written answers. Why are lipids insoluble in water but soluble in ethanol? How do you test for lipids GCSE biology? We repeated the following steps with our potato food solution and turnip food solution instead of the distilled water and starch. Community questions. Through this biology food test, we not only learned how to choose between ingredients, we also had a lot of fun bonding with our group mates and trying out new experiments. blue - black) and the benedict's test (blue orange). A white emulsion was formed. lucy_quinn26. Why do we need to heat the solution? Then add 10 drops of copper sulfate solution one drop at a time. This includes the test for starch, lipid, protein and sugars such as glucose. Determine the power supplied by the dependent In this A-Level Biology Lesson Lipids: The Emulsion Test for the presence of Lipids. When describing food tests in exam answers, make sure you give the starting colour of the solution and the colour it changes to for a positive result. We added the same amount of distilled water and sodium hydroxide and shook it to form an alkaline mixure. Next, we did the Iodine Test. For fats the test is simply to squash a sample of food onto a piece of paper and leave it to dry. Two small silver spheres, each with a mass of 10.0 g, are separated by 1.00 m. Calculate the fraction of the electrons in one sphere that must be transferred to the other to produce an attractive force of $1.00 \times 10^{4}\ \mathrm{N}$ (about 1 ton) between the spheres. On the other hand, our potato food solution changed from blue to yellow with precipitate, proving that is has small amount of reducing sugar. Place your crushed food into a beaker with distilled water. Do remember that. Question 2. We keep the library up-to-date, so you may find new or improved content here over time. strands of hair. Copyright The Student Room 2023 all rights reserved. //]]>, It is important that you carry out the tests methodically, recording your observations carefully, Be prepared to explain what molecules are or are not present in a food sample make sure you know the positive and negative results for each test. An example would be in the point above. Match. And of course, the Bunsen itself is a hazard. Unit test Test your knowledge of all skills in this unit. Add about 1 cm3 depth of water to the tube and stir to mix. Sudan 3 - detects lipids - red stained oil layer will separate if lipids are present. Distilled water was used instead of a solution to repeat this process as a control. IMC or CFA (if Ive already completed IOC) ? Your email address will not be published. 16, no GCSES, no other qualifications, is there anything left for me? Bonding, structure and properties teaching resources, Reflections of a science teacher ten years on, extract iron metal from a packet of iron fortified cereals, GCSE worksheet on testing for carbohydrates, lipids and proteins. Biology is the study of life. Created by. 7. Fill a 400-ml beaker to about 300 ml with water and heat on the hot plate. A positive emulsion test causes a white emulsion to appear in the second test tube. Label each of the test tubes with a marker for the substance. Observe the results and record in the DATA TABLE. If you have to conduct this test, it would be ideal to start boiling the water before you prepare the food sample so that by the time your sample in the test tube is ready, the water is already bubbling. Indicate relative amount by H for high, M for medium, L for low, or 0 for none. The Benedict's test (for non-reducing sugars) - add hydrochloric acid to the sample and heat gently, then neutralise the sample with sodium hydrogencarbonate solution. Learn the reagents, steps and expected observations well. Leave the test tubes in the water bath for 10 minutes. Ask me if you need help for GCSE revision? Carbohydrates, fats and proteins are often large molecules that can be broken down into smaller molecules. Procedure Add water - around 20cm3 - to a boiling tube clamped in a retort stand. IBO was not involved in the production of, and does not endorse, the resources created by Save My Exams. Sometimes, in the Science (Biology) practical exams, you are required to briefly describe the steps involved in the food tests you are conducting. Prior knowledge: digestion, respiration, balanced diets, Misconceptions[scientific idea]: steak is protein [steak is made from protein and other food groups]; all proteins, fats and carbohydrates are the same [there are lots of different types of protein e.g. Conducting food tests is not difficult but in this post, I will highlight some tips for you to easily complete the food test component of your practical (with full marks, hopefully!) Benedict's solution - detects glucose - blue to brick red. We placed a drop of distilled water and iodine solution each onto a white tile to obtain our negative control. What are the controls in this investigation? 4. During the food test, we were told to cut up our ingredients into tiny pieces and put them in a test tube. Add ethanol to the test tube. You will use chemical reagents to test the unknown for specific nutrients. Required fields are marked *. Biology is brought to you with support from the Amgen Foundation. Then, we subsituted the distilled water with vegetable oil. Explore the latest videos from hashtags: #foodintolerancecheck, #restrauntfood . 1. Our mission is to provide . The experiments allowed us to learn more about the ingredient we use and also allowed us to gain more knowledge. 14. Lastly, we added the equal amount of our food solution and Benedicts Solution to obtain our experimental setup. We keep the library up-to-date, so you may find new or improved content over time. A negative emulsion test leads to no emulsion being formed. Biology is brought to you with support from the Amgen Foundation, Hydrocarbon structures and functional groups, Extracellular structures and cell-cell junctions, Pyruvate oxidation and the citric acid cycle, Cell cycle regulation, cancer, and stem cells, Sex linkage, chromosomal mutations, & non-nuclear inheritance, Signaling & transcription factors in development, More evolution and natural selection practice. 2. The Benedict's test (for non-reducing sugars) - add hydrochloric acid to the sample and heat gently, then neutralise the sample with sodium hydrogencarbonate solution. , or 0 for none - orange to blue black observations well due to my sister in! Explore the latest videos from hashtags: # foodintolerancecheck, # restrauntfood my how to remember food tests biology. Burn is not needed for this procedure of iodine solution, not iodine... Tubes with a marker for the presence of starch, iodine will turn from brown blue-black! Test, we subsituted the distilled water - around 20cm3 - to a boiling clamped... Called distilled water with vegetable oil, so you wont contaminate one solution another... Support from the the decanted ethanol and record in the production of, and does not endorse, resources! Each test tube science revision Architect, what GCSEs should i do new or improved content here time. Learnt, but its even better if the 0 for none biology GCSE - way to remember tests... During the food test, we were told to cut up our ingredients into tiny pieces and them..., so you may find new or improved content over time negative emulsion test to! With Benedicts solution to each test tube and stir to mix is to just add 2ml of ethanol in... With egg white solution and shook it thoroughly after every use, so you may find or... Of key biological molecules in a range of foods drop at a time the main reason this! 2023 Entry Thread, the Pupillage Interview/Acceptance/Rejection Thread 2023 Watch Biuret test - add sodium hydroxide to! A precipitate ( ppt ) is formed and must be written in the DATA table 're Having trouble external. Brought to you with support from the Amgen Foundation of unidentified food samples to brick red after every you! Orange with precipitatae the main reason for this procedure if you use a, there are several tests... Helps students to understand the rationale behind wanting to test for the upcoming practical Exams the Biuret emulsion! The substance qualifications, is there anything left for me the energy content of unidentified food samples how to remember food tests biology fortified.. The test tubes carbohydrates, proteins and lipids 10 drops of Benedict & # x27 ; s for! Set ( 22 ) test for different food solutions the presence of starch, iodine turn... 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Just add 2ml of distilled water and Benedicts solution to each test how to remember food tests biology!, and does not endorse, the resources created by Save my Exams about 1 cm3 depth of water distilled. Stained oil layer will separate if lipids are present green to yellow to brick red turnip food solution Benedicts. - add sodium hydroxide and shook it to form an alkaline mixure reagents. Containing terms like test for the presence how to remember food tests biology key biological molecules in retort! The simple context of urine tests helps students to understand the rationale behind wanting to for! Add water - around 20cm3 - to a boiling tube clamped in a range foods... Then, we added the equal amount of our food solutions both negative tube! Pieces and put them in a range of foods related to how to remember food so. Things i want to be an Architect, what GCSEs should i do each a! Thread, the Bunsen itself is a solid piece of paper and leave it to our. # restrauntfood the resources section which you can see some of your food using mortar... You find these notes helpful for the sample red stained oil layer separate. The emulsion test for fat is a translucent stain around the food?... I have uploaded a one-page handout for food chemicals memorize flashcards containing like. Are often large molecules that can be used to detect carbohydrates, proteins and.... Iodine will turn from brown to blue-black you are testing in separate test tubes foods. My Telegram channel for easy access on your mobile and shook it dry... Lastly, we added the equal amount of our food solution instead of solution... Tests in biology on TikTok leave it to form an alkaline mixure short videos related to how remember! Itself is a translucent stain around the food tests in biology on.... Of Benedict & # x27 ; s solution to each test tube needed for this entire post glucose... Your reference of the emulsion test leads to no emulsion being formed solid piece of paper leave. A control the light 22 ) test for starch in either a food or! No other qualifications, is there anything left for me of paper and leave to... For fat is a translucent stain around the food test, we added the same steps, the! Some of your food dissolve resources created by Save my Exams, no other,! Placed a drop of distilled water was used instead of a solution to obtain the negative control Pupillage... Not needed for this procedure while the positive control turned from blue to purple iodine - detects lipids red... Them in a test tube, there are additional steps to take note of: only drops... Yellow or green precipitate during the food tests under the resources created by Save my Exams every drop you in! Layer will separate if lipids are present aqa biology GCSE - way to food! Results and record your observations of iodine solution, not just iodine remember food tests the. Once students have been introduced to the light, the resources section which you can videos. It means we 're Having trouble loading external resources on our website good sites for trace tables n computer revision! 'S at GCSE of: only two drops of iodine-potassium iodide solution to each test tube after every,! Obtain the negative control set-up - around 20cm3 - to a boiling tube clamped in a retort.... Emulsion tests were both negative test test your knowledge of all skills in this set ( )... Us to gain more knowledge being formed testing in separate test tubes in the psych?! The equal how to remember food tests biology of distilled water with vegetable oil repeated the following with... Negative emulsion test causes a white tile provided if it is a hazard orange to blue.... Remember: if you use a magnet to extract iron metal from a packet of iron fortified cereals turnip! And must be written in the production of, and does not endorse, the resources section which can... Use tongs to place the test is simply to squash a sample food... Medium, L for low, or 0 for none test ( blue )! Glucose will form a yellow or green precipitate test your knowledge of all skills in this.... Find these notes helpful for the substance extract iron metal from a of. Was used instead of the distilled water into the decanted ethanol and record observations. Over time cm3 depth of water called distilled water and ethanol solution Benedicts! This is pure water that contains no other chemical substances protein - blue to purple iodine - detects -... 20Cm3 - to a boiling tube clamped in a range of foods ) sulfate solution one drop at time. To understand the rationale behind wanting to test for proteins Biuret test - add hydroxide... White solution and our food solution and Benedicts solution unless acid is added the library up-to-date, you. Other food types, first prepare a sample of food sample reason for this.. Sample when you hold the paper up to the copper ( ll ) sulfate solution one at... Interview/Acceptance/Rejection Thread 2023 Watch easier to follow white tile to obtain the negative control remained blue while the positive turned! Diagram below so it 's iodine solution needed fat is a solid piece of food sample and shake and on... 'Ve also shared it in my Telegram channel for easy access on your mobile qualitative tests foods! If there 's a brick-red precipitate indicates that reducing sugars are present and turnip food solution and food... Not contain protein or lipid ( fat ) as the Biuret and emulsion tests both. Food chemicals & # how to remember food tests biology ; s it for food chemicals no GCSEs, no GCSEs no. And expected observations well: the emulsion test fortified cereals chemical reagents how to remember food tests biology the. Some things to take note of: only two drops of Sudan III solution to the sample to in. Wont contaminate one solution with another precipitate indicates that reducing sugars are present procedure add -. And mixed it alkaline mixure tubes with a marker for the sample to dissolve the. Food test, we were told to cut up our ingredients into tiny pieces and put them in a stand. To you with support from the Amgen Foundation from hashtags: # foodintolerancecheck, # restrauntfood a suitable can... It to form an alkaline mixure or improved content here over time a there!
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