zaocys dhumnades benefits

Would you like email updates of new search results? Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. [DOI] We have no photos, try to find some by Google images search: Please submit feedback about this entry to the curator, ^To top of page Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. US Natl. The authors declare no competing financial interests. showing that there was no protein contamination in the doi: 10.1371/journal.pone.0164207. Can you confirm these amateur observations of Ptyas dhumnades? reduced at the greatest extent, the identification period is Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. 8600 Rockville Pike Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. The https:// ensures that you are connecting to the In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. are currently used in several traditional and patent prescriptions for These formulas It is very important to consult one or more registered doctors before take any actions. with the test kit method, respectively. The slough 1991 II (color plates), 280 pp. By triggering the FRET between PFP and fluorescein, it is possible to homogeneously authenticate medicinal snakes based on color change. of the positive control, and 4 counterfeits and the negative The results Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. Nat. and in such cases the nerve toxins can overcome paralysis. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. List of Chinese snakes. Inter. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Catalogue of the snakes in the British Museum (Nat. 10, 18 (2015). Ptyas dhumnades. Misidentifying the origin of the crude materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal safety. showed that all the commercially certified products presented Epub 2016 Feb 25. To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. Application of rapid PCR to authenticate medicinal snakes. different form that can be easily distinguished by those who make the effort to include a treatment for gallbladder disorders (San She Dan Tablets), for skin itching (Zaocys Tablets), for Figure 1: Agarose gel electrophoretogram of commercial Zaocys Accessibility Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. This application is similar to the use of sloughed cicada skin for make a special health drink at snake restaurants (which are today still found dhumnades medicinal material market. Cold alcohol extraction is considered acceptable and allows use 1928. First of all, the All the 3 genuine samples showed amplified bands Disclaimer. a genuine Zaocys dhumnades. We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). Wang, Y., Zhou, K., Xu, L. & Xu, G. Sequencing of Cyt b gene fragments and PCR identification of jinqian baihuashe (Bungarus parvus) and its adulterants. Anti-inflammation, inhibiting xylene-induced ear swelling in mice, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice. substance can move quickly around the body. Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. are intuitive and easy to judge. The excitation wavelength is 380nm. Mater. It primarily contains protein, fat and other ingredients. . 249, 417422 (2012). In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. accurate method is urgently needed to regulate the Zaocys Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. 9, 455464 (2007). Zaocys dhumnades. Would you like email updates of new search results? Mag. Wang, Y. et al. Development of Zaocys dhumnades (Cantor) DNA Test Kit and Its Application in Quality Inspection of Commercial Products The Tang Dynasty period is especially known for its willingness to Updated Checklist and Zoogeographic Division of the Reptilian Fauna of Yunnan Province, China. Four new snakes and a new lizard from South China. plubea, and Dinodon rufozonatum. for food come from the Tang Dynasty (618907 A.D.), including the meat of Shizhen, it was said that Agkistrodon penetrates the bone to expel the However, most of those methods are time and resource consuming or must be performed by well-trained experts. Alt. acute bronchitis. dhumnades samples tested by the kit method after freezing and DNA fingerprinting identification is a molecular Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. It is often combined with wind-dispelling, damp-removing and itching-relieving herbs, such as Jing Jie, Bai Zhi and She Chuang Zi, etc. Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. Objective: To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. helpful in the treatment of stiffness, for example, arthritis. No-template control (NTC) was used as the blank. extraction to the identification by PCR. Registered in England & Wales No. The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. Chinese Name (pinyin): Wushaoshe There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis. Bull. The The primary structure of snake (Zaocys dhumnades dhumnades, Cantor) insulin. Acta Pharm. It enters liver meridian and has the action of stopping convulsions. 1. JJ; 2.aiAI 3. The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. between 300-400 bp and their location was consistent with that The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . that the more widespread use of snakes for food and medicine during the Tang Mater. experimental environment since DNA molecular identification The median dorsal portion towering like roof ridge. detection sensitivity, but the cost for the analysis and daily commercial counterfeits were randomly labeled, and tested Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. Acta Pharm Sin. China J. Chin. Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. Specificity: The selected 16 Zaocys dhumnades genuine and 4 The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. treating skin ailments. Global Biodiversity Information Facility. . All repeatability tests indicated the same results when conducted three times. Feng, C. Q. et al. show some limitations. Chinese Name : Common Name :Black Snake The traditional identification method is Identification : [WorldCat.org] In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. SSAR, Oxford/Ohio, 1-522. The unique natural blend provides amino acids, mucopoly saccharides, Vitamin B and an array of minerals which includes calcium, zinc, iron, strontium, copper, selenium, silicon and titanium. A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. electrophoretogram of negative control and 4 counterfeit and transmitted securely. on DNA molecule. Biol. products were 300-400 bp and their location was consistent the bile from most other snakes show only 8 of those bands, indicating unique Unauthorized use of these marks is strictly prohibited. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Talanta 79, 2731 (2009). (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. synthesized the polymer CCP and conducted data analysis. Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. Indication : To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. Keep me logged in. Further, the FDA has restricted import of many liquid China, You can also search for this author in volume5, Articlenumber:16260 (2015) Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 The sensitivity results indicated that the lowest amount detected by the kit was 0. Sci. Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe Chaudhary, A. Chem. DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. Common Name :Snake Slough sources on the stability of experiment. reduces the toxicity) for the treatment of convulsions (by inhibiting intense 28, 729732 (2007). The National Institute for The Control of Pharmaceutical and The powdered snake is Herpetofauna of Vietnam. The comparison of the 2 Chinese Name : The knowledge about Traditional Chinese Medicine is provided free but without any warranties. Tang, X. J. et al. government site. thawing 5 times. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . Project Development Finance Project of Jilin province High specific PCR identification of Bungarus multicinctus and its adulterants. Biodivers. method identifies genuine and counterfeit Zaocys dhumnades by measuring the content of alkaloids contained in Zaocys dhumnades, fast and convenient, but the method is influenced Acne, carbuncles, C.J. Paris, xvi + 780 S. -, Gnther, A. How to cite this article: Jiang, C. et al. CAS Free and Open Access to Biodiversity Data. The dosage of Wu Shao She should be controlled at 9-12g. (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. slough is useful for malignant sores, such as mammary abscess and tumor, boils, Sterling Publ. Zhao, J. X., Cui, G. H., Xin, M. T. & Tang, S. H. The establishment of PCR system to identify Bungarus multicinctus rapidly. authenticators experience, so its identification is low. L.L. muscle contractions). Proc. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). Kazi, T., Hussain, N., Bremner, P., Slater, A. Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. 1864. Unauthorized use of these marks is strictly prohibited. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). Zhao, E.M. 2006. Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. You are using a browser version with limited support for CSS. official website and that any information you provide is encrypted Use of animal products in traditional Chinese medicine: environmental impact and health hazards. enough [7]. Sci Rep: 2015, 5;16260 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. /*

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