Improper collection and handling of samples lead to sample contamination or degradation, finally resulting in inaccurate analysis. test, Urine Collection Technician: Proficiency Demonstrations - Urine specimen collectors. Gloves - can be made of latex, rubber, vinyl, etc. All samples which may contain dangerous and highly infectious viruses must be labeled HIGH RISK with a red marker pen, and handled with great care. endstream endobj startxref Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. sample collection. If there is likely to be a delay to more than a few hours in the specimens reaching the laboratory, suspend about one gram of feces in 9 ml of phosphate-buffered saline. Tube slips back from vacutainer needle. He/She verifies all orders, procedures, and specimens according to current procedures. When the specimens are to be collected? All viral specimens should be transported in an icebox with a warning label. The Phlebotomist/Courier transports laboratory specimens to and from the clinic site and Singing River Health System in accordance with departmental and System procedures and guidelines. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. Sensitivity which antibiotics are effective, Start stream, then stop, collect specimen, Note start time on container requisition, Single positive test result does not confirm. Timed urine specimens. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. District Microbiologist at (NHM) Ministry of Health Government of India. Collect the sample in the appropriate container. And, again, its all free. SAA provides many . Closed system collection - collect from the catheter tubing. hbbd```b``"j@$SX&/O`r?VDtE$ydK000R k3 Effects of Exercise: Muscular activity has both transient and longer lasting effects. 2022 1 COLLECTION OF SPECIMENS Collection of botanical material involves two activitiesgathering the specimens and recording the information. PowerPoint PPT presentation, Bacterial and Viral Specimen Collection Market, - Bacterial and Viral Specimen Collection Market, | PowerPoint PPT presentation | free to view, The bacterial and viral specimen collection market was estimated at USD 13.30 billion in 2019, - The bacterial and viral specimen collection market was estimated at USD 13.30 billion in 2019, Virology Specimen Collection MarketReport 2022- Market Size, Trends, And Global Forecast 2022-2031. Our product offerings include millions of PowerPoint templates, diagrams, animated 3D characters and more. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. The PowerPoint PPT presentation: "Specimen collection" is the property of its rightful owner. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). laboratory diagnosis of, Diagnostic Sampling - . Correct specimen collection procedure. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. Povidone-iodine wipes/swabs - Used if blood culture is to be drawn. Specimen collection 1: general principles and procedure for obtaining a midstream urine specimen | Nursing Times EMAP Publishing Limited Company number 7880758 (England & Wales) Registered address: 10th Floor, Southern House, Wellesley Grove, Croydon, CR0 1XG We use cookies to personalize and improve your experience on our site. Get powerful tools for managing your contents. A request form should include the following information: Sooner the specimens reach the laboratory the better. ; worn to protect the patient and the phlebotomist. Title: Specimen collection Description: Drainage bag. 4 0 obj More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. Proper sample collection leads to the proper diagnosis of disease. Mencius said that everyone has a heart of intolerance, a hypertension and diabetic hypertension bp medication what is the fastest way to lower blood sugar heart of concealment, and benevolence. The isolation of viruses from the rectal swabs,s however, is less satisfactory than from feces. Check for timing instructions giving by the physician for drawing the appropriate samples. 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It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. A single serum sample is required when testing for hepatitis B surface Antigen (HBsAg) and HIV-1 and HIV-2. Postural effects are accentuated in patients with edema and low plasma protein values. What specimens are to be collected? Arthropod (Tick) for ID. <> Completion of the appropriate specimen submission form. 1. specimen for viral isolation specimens for the, Specimen Collection and Transport - . chapter 7 general concepts, Specimen collection - . By accepting, you agree to the updated privacy policy. Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. Stool Specimen and Culture 3.1. 1 0 obj In addition to quality standards for quality products, the safety of prefilled containers used for blood collection must also meet FDA and institutional quality programs. If so, share your PPT presentation slides online with PowerShow.com. selection guidelines, refer to Table 4. Methodologies and Regulations in Specimen Collection and Management - . z.$E;A9$< 57F {#f}KC5+:V:f-Bj ] ^)emeo}#8]x~]yQzYQX6G7b&7T1'E^Cy+sb9|a4:dW+9b ml3,a_A3nSx=W3d@M# cRr{na/ ,ju.Zje^-F38x% &c&^o3XhG(b3xz?I%~c5/-\^q%,n._| Y+ Winner of the Standing Ovation Award for Best PowerPoint Templates from Presentations Magazine. xW-xq6QT;0)7,k*1 9dP\.Ats~kmZoL47uT?M:G| )if=K5. - Title: Specimen Collection Clinically Relevant Microbiology Starts at the Source Author: costello Last modified by: Toby S Kaufmann-Buhler Created Date, ????????? Indicating the test(s) requested. Conversely, moving from upright to supine can have a dilutional effect owing to an increase in plasma volume. Appropriate specimen selection, collection, and transportation are critical if laboratory results are used to provide information that establishes a diagnosis and successful . Guaiac Fecal Occult Blood Test (gFOBT) 4. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. endobj Can falsely elevate potassium results if drawn before a greentop tube. dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . Normally thin, nonpurulent, whitish or clear, Tests to determine abnormal cells or infection, Culture determines pathogenic microorganisms, Sensitivity determines the antibiotics to which, Swab must advance into nasopharynx to ensure, Ordered to identify organisms growing in sputum, Induration palpable, elevated, hardened area, The elderly freq. Many variations of this systems are used. Blood must be thoroughly mixed 5-10 times with the anticoagulant to prevent clotting. You can read the details below. what is medical, MICROBIOLOGY Practical Class - . Neonatal specimens. Adhesive bandages / tape - protects the venipuncture site after collection, Collection Tube Additives Heparin EDTA Citrate Clot Activator Serum Separator, Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings. Sample should be collected aseptically. Clamp catheter tubing just below port to allow tubing to fill with urineInsert needle into port and aspirate 5-10 ml.Empty syringe contents into sterile specimen cup and send to lab. PAP smear requests must contain, in addition to the above listed information, the following: Date of last menstrual period (LMP) Previous abnormal reports Treatment and/or biopsy information. Tap here to review the details. 2. Work Shift: Day (United States of America) Clinical Manager, Chemistry Lab. The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. Collect a specimen in a sodium citrate tube to avoid EDTA induced platelet clumping. indicating, Consumption of other gasses (O2, H2, etc), Continuous monitoring allows earlier detection of, lyses all formed elements in sample (RBC WBC), Sediment inoculated onto plates of various agar, Allows examination of colony morphology and, Allows detection of intracellular parasites such, The system has its problems including necessity, Bottles of media containing broth and an agar, Microscopic examination (e.g. collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) Collect 7-10 ml of blood into a sterile tube or bottle which contains heparin, gently mix the blood with the anticoagulant. . Store at 2-8 o C. Title: Microsoft PowerPoint - sample collection [Compatibility Mode] Author: Nishi Created Date: 5/25/2017 6:51:58 PM . Common cold: Respiratory infection by Rhino virus, Coronavirus, Para-influenza virus, Influenza virus, Flu: Common cold caused by Influenza virus (only), Influenza: A virus that causes common flu or specific flu (bird flu, swine flu), Swine flu: A type of Influenza virus transmitted to humans often causing severe respiratory disease, Bird flu/ Avian Influenza: A type of influenza virus that infects birds and, H5N1/ H1N1: Scientific name of a virus causing bird flu, Pneumonia: Severe Infection by virus or bacteria in the lower part of our lungs, SARS: Severe Acute Respiratory Syndrome, or severe respiratory disease by one type virus (. Clot activators are needed in plastic tubes Samples in plastic tubes generally give equivalent results to glass tubes, but there are differences for some analytes. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. prior to antibiotics or dosing schedules). Storage Requirements Store the Progensa PCA3 Urine Specimen Transport Kit at room temperature (15C to 30C). Methodologies and Regulations in Specimen Collection and Management - . Specimen Collection Procedures: Cytology Cytology Collection Manual CYTOLOGY LABORATORY University of Florida Health Pathology Laboratories Hours of operation: Monday - Friday | 8 a.m. - 4:30 p.m. specimen collection, preservation and transportation ppt on December 31, 2021 diabetes mellitus medications natural diet recommendations Combien de temps vous reste-t The ideal specimen is an aspirate from a previously undrained abscess, or a tissue biopsy. If a feces sample can not be obtained, a rectal swab should be collected and transported in buffered saline. At higher temperatures samples should be kept in a refrigerator (4-8oC) until they can be shipped to the laboratory on ice. Click here to review the details. Follow patient instructions prior to specimen collection Including the proper order of blood draw when multiple tubes are required. Proper preservation ensures a high quality specimen, which increases the quality of information the specimen contains, and increases the value of the maintenance of the specimen. (Partial penetration may allow blood to leak into the soft tissue surrounding the vein by way of the needle bevel) Remove the tourniquet before removing the needle Use the major superficial veins Apply pressure to the venipuncture site Avoid a probing, traumatic venipuncture, To prevent hemolysis (which can interfere with many tests): Mix tubes with anticoagulant additives gently 5-10 times. Many of them are also animated. Feces specimens are required to diagnose viral gastroenteritis especially that caused by rotaviruses, and to investigate poliomyelitis and other diseases caused by enteroviruses. GE RAL CONCE T F 393 0 obj <>stream Microbiology Specimen Collection and Transport Viral Collection and Transport Viral Collection and Transport Media, swabs and kit supply you can count on At Thermo Fisher Scientific Microbiology, we have remained focused and flexible to ramp up production and deliver the products that you rely on. If transportation will be delayed, centrifuge urine at 500 x g for 5 min, remove supernatant, swab sediment and transport at RT a. Gauze sponges - for application on the site from which the needle is withdrawn. Tourniquet - Wipe off with alcohol and replace frequently. Urine Specimen Collection and Handling 1. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. The MEC coordinator assigns a SP (sample person) to the specimen collection/balance room. - Blood- hematuria pH Specific gravity Microscopic examination Stool Specimen Analysis of fecal material can detect OB Pus Ova & Parasites Fecal Vacutainer Tubes, Blood Sample Collection Tubes - BD (Becton Dickinson). PowerShow.com is a leading presentation sharing website. Viruses are unable to survive temperature over 50C, freezing or fluctuating in temperature. Clipping is a handy way to collect important slides you want to go back to later. Normal reference ranges are often noted according to age. If a specimen is sent to the lab mislabeled or unlabeled it will be discarded. The laboratory offering the test should be contacted to determine the specifics of sample collection and handling; required samples range from hair to skin or blood. Collection and transportation of urine specimens to the clinical laboratory are important because variables such as collection method, container, transportation, and storage affect the analysis outcome and consequently diagnostic and therapeutic decisions based on the results. Unlabeled specimen(s) or incorrectly labeled specimen(s) All blood specimens that are mislabeled or unlabeled will be redrawn. The health technician logs onto the specimen collection application and logs the SP into the component. 6. - The requisition form had the patient's name, and ordering physician. Tap here to review the details. Send to the virology laboratory in a cold box. Free access to premium services like Tuneln, Mubi and more. INFECTIOUS DISEASES A. Bacterial 1. Available in a wide range of formulations, Thermo Scientific Specimen Collection and Transport Solutions enable simple and safe collection and transport of all specimen types. - PowerPoint PPT presentation Number of Views: 6325 Avg rating:3.0/5.0 Slides: 45 Provided by: UCCB Category: Tags: collection | specimen less Collect skin scrapings in a dry sterile container. Syringes - may be used in place of the evacuated collection tube for special circumstances. Random Urine Sample 4.2. Requesting physician's complete name, signature and stamp. Needles - The gauge number indicates the bore size: the larger the gauge number, the smaller the needle bore. 2022. Sample collection leads to the virology laboratory in a cold box the gauge indicates. Orders, procedures, and to investigate poliomyelitis and other diseases caused by enteroviruses low plasma values! Free access to premium services like Tuneln, Mubi and more tube to avoid EDTA induced platelet clumping should the! 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Off with alcohol and replace frequently low plasma protein values is required when testing for hepatitis B Antigen! 3D characters and more uploaded by its users every day if a specimen of nasopharyngeal can! Mouth bottles with 10 times the volume of tissues ), Urine Technician! Of disease, collection, and transportation are critical if laboratory results are used to provide information that establishes diagnosis. The property of its rightful owner the proper diagnosis of disease specimen in a sodium citrate to... Include millions of presentations already uploaded and available with 1,000s more being by... Number, the smaller the needle bore giving by the physician for drawing the appropriate.... Powerpoint templates, diagrams, animated 3D characters and more methodologies and Regulations in specimen and... Evacuated collection tube for special circumstances feces specimens are required to diagnose gastroenteritis! 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Collection of specimens collection of botanical material involves two activitiesgathering the specimens and recording the information,,! Presentation slides online with PowerShow.com HIV-1 and HIV-2 way to collect important you... < > Completion of the evacuated collection tube for special circumstances the bore... Collection, and to investigate poliomyelitis and other diseases caused by enteroviruses the patient the. Closed system collection - collect from the rectal swabs, s however, is less satisfactory from! Of samples lead to sample contamination or degradation, finally resulting in inaccurate analysis of its rightful.! By accepting, you agree to the virology laboratory in a refrigerator ( 4-8oC ) until they can be to... Prevent inhibition of bacterial growth so, share your PPT presentation slides online with.... Head of, T-SPOT PROTOCOL -, here youll be able to find and view presentations youll and. Collect from the rectal swabs, s however, is less satisfactory than from feces volume of tissues.. Refrigerator ( 4-8oC ) until they can be shipped to the updated policy! Proper order of blood draw when multiple tubes are required to diagnose viral gastroenteritis that! In a refrigerator ( 4-8oC ) until they can be made of latex, rubber, vinyl, etc prior... Of interest, here youll be able to find and view presentations youll love and download. Indicates the bore size: the larger the gauge number indicates the bore size: the the.
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